OK-432联合IL-2抑制C_(57)BL/6小鼠Lewis肺癌的生长及PCNA的表达

OK-432 combination IL-2 inhibiting C_(57)BL/6 mice bearing Lewis lung cancer growth and the PCNA expression

目的：研究OK－432与IL－2抑制C_（57）BL／6小鼠Lewis肺癌（LLC）生长及增殖细胞核抗原（PCNA）的表达。方法：40只C_（57）BL／6荷瘤LLC小鼠，雌雄各半，分4组，分别用生理盐水、OK－432与IL－2处理，观察用药后对肿瘤生长的抑制及用抗鼠PCNA单克隆抗体进行SABC免疫组化技术半定量测定PCNA在Lewis肺癌原发灶中的表达。结果：二者联用后明显增强抑癌率（P＜0．05），对肿瘤体积的抑制比二者单独用药更明显（P＜0．01），PCNA在用药组中表达与对肿瘤生长抑制情况一致，二者联用后增殖活性明显下降（P＜0．01）。结论：OK－42与IL－2有明显的协同抗肿瘤活性，PCNA可作为抑制肿瘤生长的一项有用的生物学行为指标。

Objective: To study OK-432 and IL-2 inhibition C_(57)BL/6 mice bearing Lewis lung cancer (LLC) growth and proliferating cell nuclear antigen (PCNA) expression. Methods: 40 C_(57)BL/6 mice bearing LLC, half male and half female, were divided 4 groups, and injected with NS, OK-432 and IL-2. The tumor growth inhibition afte drug administration was observnd and the monoclonal antibody was used to PCNA and SABC immunohistochemical method the expression of PCNA in paraffin-embedded primary tumor tissues were semiquantitatively determined. Results: Combination administration group significantly increased inhibition rate (P<0.05), and enhanced inhibition effect on tumor volume versus control (P<0.01 ), whose anti-tumor effect was more significant than administration alone, the expression of PCNA was consistent with inhibition tumor growth, proliferating activity obviously decreased (P< 0.01 ). Conclusion:oK-432 and IL-2 had distinctiy synergetic anti-tumor effect, and PCNA may be a useful index for studying them inhibition tumor growth.