运动对大鼠学习记忆功能和海马CA_3区HDAC_2表达的影响

The effect by exercise on function of Rat's learning and memory as well as HDAC_2 expression in hippocampal CA_3 region

目的:观察中等强度、递增负荷的跑台运动对大鼠学习记忆功能和海马CA3区HDAC2表达的影响。方法:将生长发育期雄性大鼠随机分为对照组和运动组,运动组大鼠进行8周中等强度、递增负荷的跑台训练。8周后用Morris水迷宫法检测两组大鼠学习记忆能力,检测完后处死两组大鼠,在大脑海马CA3区取材,用免疫组织化学法检测大鼠大脑海马CA3区HDAC2蛋白表达。结果:定位航行实验中,运动组大鼠逃避潜伏期与对照组相比明显减少(P<0.01);空间探索实验中运动组大鼠在目标象限停留时间,穿越平台次数与对照组相比明显增加,且差异显著(P<0.01)。运动组HDAC2蛋白表达与对照组比较明显下调(P<0.05)。结论:中等强度运动能促使大鼠大脑海马CA3区HDAC2表达减少,说明长期适宜的体育运动能通过抑制HDAC2表达,提高学习与记忆能力。

Objective：To reveal the effect on function of rat’s learning and memory as well as HDAC2 expression in hippocampal CA3 region by moderate and incremental treadmill exercise.Methods：Male rats in developing period were randomly divided into control and exercise groups.Rats in latter group underwent incremental moderate exercise on treadmill for 8 weeks.The Morris water maze was then immediately employed to verify learning and memory of rats in both groups before being executed.Materials drawn from the hippocampus CA3 region were then processed by immunohistochemistry to assess CA3 HDAC2 protein expression.Results：It is found in navigation experiment that the escape latency of rats in exercise group was significantly reduced compared with that of the control group（P 〈0.01）.Both the diration of target quadrant movement and the platform-crossing number of rats in exercise group increased significantly compared that of control group with difference being statistically significant（P 〈0.01）.Meanwhile,HDAC2 protein expression in exercise group was significantly lower than that of control group（P 〈0.05）.Conclusion：The exercise in moderate-intensity can cause the diminished hippocampal CA3 HDAC2 expression in rat’s brain.It indicates that the improvement of learning and memory can be realized through appropriate long-term exercise which make possible inhibition of HDAC2 expression.