参环毛蚓脂类挥发性成分分析

Study on Lipids and Other Volatile Constituents in Pheretima aspergillum

为了分析参环毛蚓(Pheretimaaspergillum)的脂类成分,我们采用不同溶剂索氏提取法、从该药材得到三种组分,用GC-MS法进行测定,确定了36种化合物的结构。在这三种组分中,乙醚组分中的11种成分均为脂类,不饱和脂肪酸的含量最高,占27.70%,如油酸、亚油酸、花生四烯酸和花生三烯酸。丙酮组分的脂类成分8种,占35.75%,不饱和脂肪酸只有亚油酸一种;乙醇组分的脂类成分13种,占72.09%,没有发现不饱和脂肪酸。本研究确定了参环毛蚓脂肪酸,尤其是不饱和脂肪酸的化学组成,为该中药化学物质基础的研究与药物的开发利用提供了依据。

To study chemical constituents in Pheretima aspergillum,three kinds of fractions were obtained from this drug by soxhlet extraction with different solvents,and the chemical structures of thirty-six volatile components were identified by means of GC-MS.The eleven in ether fraction were all lipids and the relative content of non-saturated fatty acid was the highest(27.70%) such as oleic acid,linoleic acid,arachidonic acid and eicosatrienoic acid;There were eight lipids in acetone fraction (35.75%), which included one kind of nonsaturated fatty acid (linoleic acid);There were thirteen lipids in ethanol fraction (72.09%),which non-saturated fatty acid has never been detected.This study has determined the lipid composition in Pheretima aspergillum,espe- cially non-saturated fatty acid,and afforded chemical base to cardio-cerebro-vascular therapy.