摘要
目的研究内源性雌激素对糖尿病小鼠血糖、血清胰岛素水平和抗氧化能力的影响,探讨内源性雌激素对胰岛细胞和胰岛素敏感性可能的保护作用。方法小鼠按体重随机分为4组,假手术组(Sham)、去卵巢手术组(Ovx)、假手术注射链脲佐菌素(streptozotocin,STZ)组(Sham+STZ)、去卵巢手术注射STZ组(Ovx+STZ)。切除小鼠两侧卵巢,腹腔注射STZ制糖尿病小鼠模型。跟踪测定体重、血糖,进行糖耐量试验。取血制血清,取出胰腺于4%多聚甲醛中同定。测定血清中胰岛素浓度,丙二醛(MDA)、总抗氧化力(T.AOC),所取胰腺做病理切片,观察胰岛细胞的变化。结果Ovx组血糖和MDA值明显高于Sham组.总抗氧化力低于Sham组;Ovx+STZ血糖和MDA值高于Sham+STZ组,胰岛素水平和T。AOC要低于Sham+STZ组。结论内源性雌激素对糖尿病小鼠具有一定保护作用。
Objective To investigate the effects of endogemms estrogen on blood glucose level, serum insulin level, and plasma total antioxidant capacity in streptozotocin-induced diabetic female mice, and to explore possible proteetive effects of estrogen on pancreatic islet cells. Methods Female mice were randomly according to body weight divided into four groups : ( 1 ) Sham ( Sham operation and vehicle administration ) ; ( 2 ) Ovx ( ovariectomy and vehicle administration ) ; ( 3 ) Sham + STZ ( Sham operation and STZ administration ) ; and ( 4 ) Ovx + STZ ( ovariectomy and STZ administration). The diabetic mice were induced by intraperitoneal injection of streptozotocin (STZ, 50 mg/kg). Blood glucose levels were measured once a week. Results The blood glucose level and mahmdialdehyde of Ovx group were higher than that in Sham group, while total anti-oxidant capacity ( T-AOC ) was lower than those in Sham group, the blood glucose level and MDA of Ovx+ STZ group were higher than those in Sham +STZ group, while T-AOC and serum insulin level were lower than those in Sham + STZ group. Conclusions Endogenous estrogen may have some protective effects on pancreatic islet function from streptozotocin-indueed diabetes mellitus in female mice.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2012年第6期501-504,共4页
Chinese Journal of Endocrinology and Metabolism
基金
甘肃省科技厅自然科学基金项目(1010RJZA111)
甘肃省中医药科研项目(GZK-2010-38)
关键词
雌激素
糖尿病
实验性
抗氧化力
胰岛素
Estrogen
Diabetes mellitus, experimental
Antioxidant capacity
Insulin