内源性雌激素对STZ诱导雌性小鼠糖尿病的保护作用研究

Protective effect of endogenous estrogen on streptozotocin-induced diabetes mellitus in female mice

目的研究内源性雌激素对糖尿病小鼠血糖、血清胰岛素水平和抗氧化能力的影响，探讨内源性雌激素对胰岛细胞和胰岛素敏感性可能的保护作用。方法小鼠按体重随机分为4组，假手术组（Sham）、去卵巢手术组（Ovx）、假手术注射链脲佐菌素（streptozotocin，STZ）组（Sham＋STZ）、去卵巢手术注射STZ组（Ovx＋STZ）。切除小鼠两侧卵巢，腹腔注射STZ制糖尿病小鼠模型。跟踪测定体重、血糖，进行糖耐量试验。取血制血清，取出胰腺于4％多聚甲醛中同定。测定血清中胰岛素浓度，丙二醛（MDA）、总抗氧化力（T．AOC），所取胰腺做病理切片，观察胰岛细胞的变化。结果Ovx组血糖和MDA值明显高于Sham组．总抗氧化力低于Sham组；Ovx＋STZ血糖和MDA值高于Sham＋STZ组，胰岛素水平和T。AOC要低于Sham＋STZ组。结论内源性雌激素对糖尿病小鼠具有一定保护作用。

Objective To investigate the effects of endogemms estrogen on blood glucose level, serum insulin level, and plasma total antioxidant capacity in streptozotocin-induced diabetic female mice, and to explore possible proteetive effects of estrogen on pancreatic islet cells. Methods Female mice were randomly according to body weight divided into four groups ： （ 1 ） Sham （ Sham operation and vehicle administration ） ; （ 2 ） Ovx （ ovariectomy and vehicle administration ） ; （ 3 ） Sham ＋ STZ （ Sham operation and STZ administration ） ; and （ 4 ） Ovx ＋ STZ （ ovariectomy and STZ administration）. The diabetic mice were induced by intraperitoneal injection of streptozotocin （STZ, 50 mg/kg）. Blood glucose levels were measured once a week. Results The blood glucose level and mahmdialdehyde of Ovx group were higher than that in Sham group, while total anti-oxidant capacity （ T-AOC ） was lower than those in Sham group, the blood glucose level and MDA of Ovx＋ STZ group were higher than those in Sham ＋STZ group, while T-AOC and serum insulin level were lower than those in Sham ＋ STZ group. Conclusions Endogenous estrogen may have some protective effects on pancreatic islet function from streptozotocin-indueed diabetes mellitus in female mice.