摘要
用构建好的重组质粒转化枯草芽孢杆菌(Bacillus subtilis)WB600感受态细胞,进行不同时间预培养后,涂布于抗生素平板培养,研究一种更高效的转化方法。用载体与外源片段的连接产物转化WB600感受态细胞,进行最佳预培养时间培养后,涂布于抗生素平板培养,培养得到的转化子用PCR快速验证和双酶切鉴定,研究一种转化子的快速验证方法。
In order to study an effective method for transformation, Bacillus subtilis WB600 competent cells, transformed with pre-built recombinant plasmids, were precultured with different period of time and smeared to the plates containing antibiotics. In order to construct a fast and effective method for transformants verification, WB600 competent cells were transformed with vector and foreign DNA, preincubated with the optimal time, and smeared to the plates containing antibiotics. The transformants were verified with PCR and double restriction enzyme digestion.
出处
《广西科学院学报》
2012年第2期117-119,共3页
Journal of Guangxi Academy of Sciences
基金
广西自然科学基金重点项目(2010GXNSFD013030)
广西培养新世纪学术和技术带头人专项资金项目
广西科学研究与技术开发课题项目(桂科攻11107008-4)资助
关键词
枯草芽孢杆菌
转化方法
转化率
感受态细胞
Bacillus sub tilis , transformation approach, transformation efficiency, competent cell
