射干提取物抗雌激素缺乏大鼠骨质疏松的研究

Anti-osteoporosis of different extracts from Belamcandae Rhizoma in estrogen deficiency rats

目的观察射干不同提取物对卵巢切除致大鼠骨质疏松症的影响,筛选活性部位。方法 96只雌性SD大鼠随机分为8组,第1组切去腹腔中少量脂肪作为假手术组;第2至8组均切除双侧卵巢,第2组作为模型组,第3至8组作为给药组。6个给药组中,2组分别ig射干总黄酮100、20mg/kg,2组ig射干乙醇提取物197、40mg/kg,2组ig射干苷15.30、3.06mg/kg,连给4个月,测定骨矿密度(BMD),骨矿含量(BMC),血清钙、磷的水平和AKP活性。结果射干总黄酮、射干醇提取物能明显改善大鼠因雌激素缺乏引起的骨矿丢失,提高BMD和BMC,改善骨骼力学性能。结论射干总黄酮提取物具有良好的抗骨质疏松症的效果。

Objective To investigate the effects of different extracts from Belamcandae Rhizoma on osteoporosis in rats after ovariectomy（OVX）.Methods Ninety-six SD female rats were randmly divided into eight groups.The control group had a sham-operation by cutting adipose tissue.The model group had OVX,while the rest six groups received bilateral OVX as drug groups.Animals in drug groups were respectively ig administrated with different extracts from Belamcandae Rhizoma for four months：two groups of the total flavone extract（100 and 20 mg/kg）,two groups of the ethanol extract（197 and 40 mg/kg）,and two groups of the tectoridin（15.30 and 3.06 mg/kg）.The levels of bone mineral density（BMD）,bone mineral content（BMC）,calcium and phosphorus in serum,and the activation of AKP were observed in each group.Results Compared with the model group,the total flavone extract and the ethanol extract of Belamcandae Rhizoma could significantly improve the loss of bone mineral induced by estrogen deficiency,increasing BMD and BMC,and promote the skeleton mechanical property.Conclusion The total flavone extract from Belamcandae Rhizoma has a distinct and preventive effect on osteoporosis rats.