Survivin反义核苷酸对宣威肺腺癌移植瘤裸鼠模型作用的研究

Study on effect of survivin antioligonucleotide on subcutaneous transplanted Xunwei lung adenocarcinoma nude mice model

目的选用宣威肺腺癌细胞裸鼠移植瘤模型,采用survivinmRNA的反义寡核苷酸(ASODN)抑制宣威肺腺癌细胞,观察其疗效,为进一步临床研究提供实验依据。方法建立宣威肺腺癌细胞XWLC-05裸鼠皮下移植瘤模型,选取48只成瘤裸鼠,随机分为:对照组、单纯脂质体转染(LIP)组、survivin正义寡核苷酸(SODN)组、survivinASODN组,通过皮下移植瘤内多点注射后,观察裸鼠一般情况、移植瘤体积,计算抑瘤率等。病理学检测各组肿瘤的变化以及对心脏、肝脏、肾脏的影响。结果 sur-vivinASODN组注射survivinASODN后,裸小鼠表现为生长速度较为缓慢,质量低于其他3组(P>0.05),其生长抑制率高于其他3组(P>0.05)。而对照组、LIP组、survivinSODN组在肿瘤质量及生长抑制率方面均无明显差异。病理检查见瘤组织中有大量的细胞坏死灶。各组裸鼠均未出现死亡,病理检查重要脏器未见明显损害。结论 survivinASODN能够抑制裸鼠皮下移植瘤的生长,未对其他脏器产生明显损害。

Objective By selecting transplanted Xunwei lung adenocarcinoma nude mice model, to adopt survivinnmRNA antisense oligonucleotide to inhibit Xunwei lung adenocarcinoma cells and to observe its effects to provide the experiment evidence for further clinical study be offered in clinical treatment. Methods After modeling subcutaneous transplanted Xunwei lung adenocarcinoma cell XWLC-05 nude mice,48 nude mice were selected and devided into 4 groups randomly： control group （blank）, Lip group （simple liposome）, survivin-SODN group （injecting sense oligonucleotide） and survivin-ASODN group （injecting antisense oligo nucleotide）. We injected different drugs according to different groups. Then the general activity of nude mice in different groups was observed and the tumor size was measured. Also, the antineoplastic rates in different groups were calculated. The pathological methods were used to analyse the effects of 4 groups on heart, liver and kidney under different treatmen. Results In the Lip-ASODN group, the tumor size was increased slowly and weight was lower than that of the other 3 groups. The antineoplastic rate in the Lip- ASODN group was significantly higher than that of other groups（P^0.05）. However, as time passed, the tumor size was increased in the control group, Lip group and survivin-SODN group, there was no statistical difference among these 3 groups（P〉0.05）. A great quantity of cell necrosis were seen under microscope. Death happened in all groups. The pathological examination revealed no obvious damage in vital organs（heart,liver,kidney）. Conclusion Survivin antioligonucleotid could prohibit the growth of subcuta neous transplanted Xunwei lung adenocarcinoma in nude mice with little damage on vital organs.