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肿瘤相关巨噬细胞microRNA表达谱及生物信息学分析 被引量：3

Profile of microRNA expression in tumor associated macrophage and bioinformatics analysis
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摘要 目的研究肿瘤相关巨噬细胞(TAM)microRNA的表达谱。方法建立小鼠乳腺癌细胞系4T1移植瘤模型,从移植瘤组织中分离TAM,用基因芯片检测microRNA表达谱,实时荧光定量PCR(real-time PCR)验证芯片结果并进行生物信息学分析,以小鼠腹腔巨噬细胞(PEC)为阴性对照。结果与阴性对照细胞相比,TAM中有59个mi-croRNAs表达量出现显著变化,其中23个microRNAs表达上调,有36个microRNAs表达下调;实时荧光定量PCR对miR-146a、miR-222、miR-31和miR-877的表达进行了验证,其结果与基因芯片检测结果一致;这些microRNAs参与了多个信号通路的调控。结论 microRNA表达谱及生物信息学分析表明microRNA在TAM分化过程的调控中有重要作用。 Objective To investigate the profile of microRNA expression in tumor associated macrophage （TAM）. Methods An xenograft mouse model was established with mouse breast cancer cell line 4T1. TAM were isolated from the tumor tissue. The microRNA expression profile was detected by using a microRNA chip assay. The result of chip assay was validated by real-time PCR and analyzed by bioinformatics. The peritoneal macrophage was used as control. Results There were significant changes in 59 microRNAs＇ expression in TAM as compared with the negative control. Among these microRNAs, 23 microRNAs＇ expression was up regulated and 36 were down regula- ted. Real-time PCR verified the expression of miR-146a, miR-222, miR-31 and miR-877, these results are in line with chip experiment. These microRNAs participate in the regulation of various signaling pathways. Conclusions Profile of microRNA expression and bioinformatics analysis suggeste microRNA plays an important role in the regu- lation of TAM differentiation.
作者 雷宇 刘彦信 葛晔华 史娟 郑德先
机构地区 中国医学科学院 基础医学研究所 医学分子生物学国家重点实验室
出处 《基础医学与临床》 CSCD 北大核心 2012年第7期767-772,共6页 Basic and Clinical Medicine
基金 国家自然科学基金(30972684 2699 232263)
关键词 MICRORNA 肿瘤相关巨噬细胞 基因芯片 生物信息学 microRNA tumor associated macrophage chip bioinformatics
分类号 R730.2 [医药卫生—肿瘤]
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参考文献12

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共引文献6

同被引文献41

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引证文献3

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基础医学与临床
2012年 第7期

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