牛血液白细胞源抗菌肽的纯化及活性鉴定

Purification and activity identification of antimicrobial peptides from bovine white blood cell

为了分离纯化牛白细胞中的抗菌肽(Antimicrobial peptides,AMPs)并对其体外抗菌活性进行初步分析,以中国荷斯坦奶牛血液为材料,通过离子交换层析法纯化抗菌肽,用反相高效液相色谱法(RP-HPLC)进一步纯化,用双层琼脂糖弥散法测定其抗菌活性。结果表明,通过离子交换层析获得的阳离子峰有抗菌活性,通过RP-HPLC对阳离子峰纯化后获得了6个峰;6个峰液对大肠杆菌和沙门氏菌均具有抗菌活性,峰液F1、F2和F3对金黄色葡萄球菌具有抗菌活性,仅峰液F1对白色念珠菌具有抗菌活性。

Antimicrobial peptides were isolated and purified from bovine White blood cell, and their antimicrobial activity in vitro was analyzed. Antimicrobial peptides from the White blood cell of Chinese Holstein were purified by ion exchange chromatography and purified by RP-HPLC. Antimicrobial activity of purified peptide was tested by the agarose radial diffusion assay. The result showed that the positive ion peak acquired by ion exchange chromatography had antimierobial activity. Six peaks acquired by RP-HPLC had the activity against E coli and Salmonella, peak F1, peak F2 and peak F3 had the activity against Staphylococcus aureus, only peak F1 had the activity against Candida albicmls.