新型小片段基因表达载体构建方法

A Novel Method for Constructing Small Fragment Gene of Expression Vector

介绍一种新型快速构建小片段基因表达载体的方法。该方法省去了目的基因酶切和酶切后纯化步骤,并省去了载体酶切后胶回收纯化步骤,具有简单快速,节省试剂费用,连接效率高等特点。应用该方法已经完成了4个小片段基因(67bp)表达载体的构建。

A new method for rapid constructing small fragment of expression vector is introduced. This method eliminates the need for the target gene digested with restriction enzyme and recovery steps, and eliminates the vector gel purified steps after digestion. It is simple and fast, saving reagent costs, high connection efficiency. Four expression vectors of small fragment gene（67bp） have been completed with this method.