RECK和MMP-9在子宫内膜癌组织中的表达及其临床意义

Expressions of RECK and MMP-9 in endometrial carcinoma and their clinical significance

目的:检测子宫内膜癌(endometrial carcinoma,EC)组织中RECK(reversion-inducing cysteine-rich protein with Kazal motifs)mRNA和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)mRNA的表达情况,探讨两者在子宫内膜癌中的临床意义。方法:选取2009年3月至2010年11月在滨州医学院附属医院妇科接受手术的子宫内膜癌患者组织标本42例,应用定量PCR法检测RECK mRNA及MMP-9 mRNA在子宫内膜癌中的表达情况,分析其相关性和临床意义。结果:在正常增生期子宫内膜、子宫内膜不典型增生、子宫内膜癌组织中RECK mRNA表达水平依次降低[(6.30±0.34)、(4.29±0.36)、(0.24±0.18),F=427.35,P<0.05],MMP-9 mRNA依次升高[(0.08±0.82)、(5.04±0.30)、(6.22±0.32),F=1117.52,P<0.05],RECK和MMP-9 mRNA的表达与子宫内膜癌临床分期、分化程度、淋巴转移关系密切(P<0.05),在子宫内膜癌中,MMP-9mRNA和RECK mRNA之间存在负相关(r=-0.478,P<0.01)。结论:定量PCR检测RECK mRNA和MMP-9 mRNA对子宫内膜癌早期诊断及预测癌前病变风险有一定参考价值。

Objective： To investigate the expressions of reversion-inducing cysteine-rich protein with Kazal motifs（RECK） mRNA and matrix metalloproteinase-9（MMP-9） mRNA in endometrial carcinoma and explore their clinical significance.Methods： Forty-two endometrial carcinoma samples were obtained from gynecological surgery in the Affiliated Hospital of Binzhou Medical University（Mar.2009 to Dec.2010）.Real-time quantitative PCR was used to assess the expressions of RECK and MMP-9 mRNA in endometrial carcinoma,and their relationship with clinic pathological features of endometrial carcinoma was analyzed.Results： The expression level of RECK mRNA was progressively decreased from the normal to invasive carcinoma（[6.30 ± 0.34],[4.29 ± 0.36],[0.24 ± 0.18],F = 427.35,P 0.05）,while MMP-9 mRNA progressively increased（[0.08 ± 0.82],[5.04 ± 0.30],[6.22 ± 0.32],F = 1117.52,P 0.05）.Both expressions of RECK and MMP-9 mRNA were correlated with TNM stage,histological grade and lymph node metastasis of endometrial carcinoma（P 0.05）.There existed a significantly negative correlation between the expressions of RECK mRNA and MMP-9 mRNA in endometrial carcinoma（r =-0.832,P 0.01）.Conclusion： Combined detection of RECK mRNA and MMP-9 mRNA by quantitative PCR has a certain value for predicting risk of early diagnosis of endometrial carcinoma and precancerous lesions.