金属基质蛋白酶MMP3和MMP7在小鼠药理性孕酮撤退月经模型中的定位

Localization of matrix metalloproteinases MMP3 and MMP7 in a mouse menstrual-like model created by pharmacologic progesterone withdrawal

目的本研究旨在研究金属基质蛋白酶(MMPs)在小鼠药理性孕酮撤退月经模型中的定位。方法卵巢切除的小鼠在诱导蜕膜化之后给予米非司酮处理,免疫组化检测金属基质蛋白酶MMP3和MMP7定位。结果 MMP3从米非司酮处理后0 h到24 h,存在于腔上皮下的局部基质、上皮、蜕膜化区域以及基底层和功能层的交界处;从32 h到48 h存在于腔上皮以及其下基质区域。MMP7从0 h到24 h存在于基底层,上皮和中间蜕膜化区域,从32 h到48 h与MMP3的定位相一致。结论 MMP3和MMP7蛋白质在此模型中定位呈区域性分布。

Objective： To investigate the expression of matrix metalloproteinases （MMPs） in a mouse menstrual- like model created by pharmacologic progesterone withdrawal. Methods： The menstrual-like model was prepared by administration of mifepristone after decidualization of ovariectomized mice. Immunohistochemistry was performed to detect the locations of MMPs proteins in the uterus. Results： MMP3 was present in the focal subluminal stromal zone, epithelium, decidual zone and interface between the basal zone and functional zone from 0 h to 24 h after administration of mifepristone, and epithelium and subluminal epithelium stroma from 32 h to 48 h. MMP7 was localized in the basal zone, epithelium and mid decidual zone from 0 h to 24 h, and a similar localization to MMP3 from 32 h to 48 h.Conclusions. The localization of MMP3 and MMP7 proteins in the model displayed a regional characterand functioned in endometrium repair, which was consistent with those previously reported data in the mouse model by physiological progesterone withdrawal and in primates.