抗氧化剂半胱氨酸对小鼠卵母细胞体外成熟及发育潜能的影响

Effects of anti-oxidant cysteine on in vitro maturation and development potential of mouse oocytes

目的研究抗氧化剂半胱氨酸对小鼠卵母细胞体外成熟及发育潜能的影响,为进一步优化卵母细胞体外成熟培养体系提供理论依据。方法对照组采用基础体外培养液,共培养101枚GV期小鼠卵母细胞;实验组采用基础体外培养液+0.6 mmol/L半胱氨酸,共培养115枚GV期小鼠卵母细胞。观察两组卵母细胞的成熟率、谷胱甘肽的含量和线粒体内膜电位。结果对照组和实验组卵母细胞的成熟率分别为68.3%和80.0%,差别具有统计学意义(P<0.05);对照组和实验组卵母细胞平均谷胱甘肽含量分别为0.48±0.6μg/L和0.51±0.5μg/L,实验组谷胱甘肽含量略高,但两者无统计学差异(P>0.05)。线粒体内膜电位,对照组与实验组分别为0.04±0.003△Ψm与0.39±0.020△Ψm,实验组线粒体内膜电位明显高于对照组(P<0.05)。结论抗氧化剂半胱氨酸对体外培养小鼠卵母细胞成熟有一定的促进作用,并可能会提高卵母细胞的发育潜能。

Objective： To investigate the effects of anti-oxidant cysteine on in vitro maturation and development potential of mouse oocytes for further optimizing the system of oocytes matured in vitro. Methods： One hundred and one GV oocytes of mouse were cultured in the in vitro culture based medium which consisted of HTF ＋0.075 IU/ml HMG＋0.5 IU/ml hCG ＋1 9g/ml E2in control group. One hundred and fifteen GV oocytes of mouse were cultured in the medium mentioned above plus 0.6 mmol/L cysteine in the experimental group. The oocytes in both groups were cultured for 16- 18 hours, and then the maturation rates, glutathione levels and mitochondrial membrane potential were observed. Results： The oocytes maturation rate in the experimental group 80. 0% （92/115） was significantly higher than that in the control group [-68.3% （69/101） ]（P〈0.05）. The average glutathione levels in the content of oocytes of the experimental group were 0.51± 0.5 μg/L and slightly higher than those in the control group （0.48±0.6 μg/L） but there was no statistically significance （P〉0.05）. The mitochondrial membrane potential in the experimental group was 0.39 ：k 0.02AXIrm and significantly higher than that in the control group （0.04±0. 003△ψm） （P〈0.05）.Conclusions： Anti-oxidant cysteine played a significant protective effect on in vitro maturation and development potential of mouse oocytes.