ENTPD5在急性白血病骨髓液中的表达

Expression of ENTPD5 in bone marrow of acute leukemia

目的观察内质网酶ENTPD5蛋白在急性白血病(AL)骨髓液中的表达,了解其与AL发病的关系。方法选取AL患者111例,其中急性髓系白血病(AML)患者78例(初治组48例,复发组10例,缓解组20例),急性淋巴细胞性白血病(ALL)患者33例(初治组14例,复发组9例,缓解组10例)。将62例初治AL患者分为高危组26例,中危组19例,低危组17例。应用Western blot法检测ENTPD5在AL中的表达量,比较在不同分型AL患者中初治、复发、缓解阶段以及在低危、中危、高危组AL患者中该蛋白表达的差异;同时分析ENTPD5表达与临床指标的相关性。结果ENTPD5的表达水平在AML、ALL患者中初治和复发组均高于缓解组(P<0.05),低危组ENTPD5的表达量明显低于高危组和中危组(P<0.05)。ENTPD5的表达量与初治时外周血白细胞计数、骨髓原始细胞百分比、基因WT1和微小残留病灶均有一定相关性。结论 AL存在ENTPD5的表达异常,该蛋白在AL的发生中可能起重要作用。

Objective To investigate the expression of the endoplasmic reticulum （ER） enzyme ENTPD5 in acute leukemia （ AL）, and to elucidate its role in the pathogenesis of AL. Methods 111 patients with AL： 78 AML cases （48 denovo cases, 10 relapse cases, 20 AML-CR cases） , 33 acute lymphocytic leukemia （ALL） cases（ 14 denovo cases, 9 relapse cases, 10 ALL-CR cases） ; 62 denovo patients with AL： 26 high-risk cases, 19 standard-risk cases, 17 low-risk cases. ENTPD5 expression levels in different groups of AL were detected by Western blot, com- parison in different groups of AL and in low-risk, standard-risk, high-risk patients with AL amount of protein ex- pression, analyzed its relativity with clinical parameters. Results ENTPD5 expression levels were higher in denovo and relapse AML cases than those in AML-CR cases, the same result with ALL cases （P 〈 0.05 ）. There was dif- ferent protein expression in different risk stratification of patients, the expression of ENTPD5 was lower in low-risk patients than that in high-risk and standard-risk cases （P 〈 0. 05 ）. ENTPD5 expression had certain relativity with peripheral blood leukocyte count and the proportion of progenitor cells bone marrow in pathogenesis, gene WT1 and MRD. Conclusion There is abnormal expression of ENTPD5 in AL, the ER enzyme ENTPD5 plays important roles in the development of AL.