急性心肌梗死患者血清C反应蛋白与基因T-757C多态性的相关性

Relationship between serum CRP leve and gene T-757C polymorphism in patients with acute myocardial infarction

目的:探讨中国苏南地区汉族人群血清C反应蛋白(CRP)水平与急性心肌梗死(AMI)发病风险及CRP基因T-757C多态性的相关性。方法:对213例AMI患者(AMI组)和173例非冠心病者(对照组)采用聚合酶链反应-限制性片段长度多态性技术检测CRP基因T-757C多态性,并采用酶联免疫吸附法检测血清CRP水平。结果:①两组患者都存在CRP基因T-757C多态性;②AMI组CRP血清水平(5.985/6.527μg/ml)与对照组(3.262/1.356μg/ml)之间差异有统计学意义(P<0.01),且经校正性别、年龄、吸烟、高血压、糖尿病和血脂异常等因素后,仍差异有统计学意义(P<0.01);③AMI组CRP基因T-757C位点CC、TC和TT 3种基因型中任意两种之间血清CRP水平均差异无统计学意义,且同性别和同年龄段亚组的该位点任何基因型之间也差异无统计学意义。结论:中国苏南地区汉族人群血清CRP水平不受CRP基因T-757C多态性的影响。

Objective：To explore the relationship between serum C-reactive protein（CRP） level and CRP gene T-757C polymorphism in Chinese Han population of Sunan region. Method：The CRP gene T-757C polymorphism was genotyped by polymerase reaction restriction-fragment length polymorphism analysis,and the serum CRP level was measured by enzyme-linked immunosorbent assay in 213 AMI patients（AMI group） and 173 subjects without coronary heart disease（CHD）（control group）. Result：①There were CRP gene T-757C polymorphisms in both groups;②The serum CRP level in AMI group（5.985/6.527 μg/ml） was significantly higher than control group（3.262/1.356 μg/ml）（P〈0.01）,and there were still significant differences after adjusting for gender,age,smoking,hypertension,diabetes and dyslipidemia（P〈0.01）;③There was no significant difference about the serum CRP level among any genotypes（CC,TC or TT） of the CRP gene T-757C（4.069/2.493 pg/ml vs 5.745/6.321 pg/ml vs 6.127/6.629 pg/ml）in AMI group,and there was also no significant difference in the serum CRP level among any genotypes in this locus within the same gender and age. Conclusion：The CRP gene T-757C polymorphism is irrelevant to the serum CRP level in Chinese Han population of Sunan region.