骨涎蛋白在不同类型种植体骨界面改建中的变化

Expression of bone sialoprotein in the process of implant-bone interface remodeling with different implants

目的:比较埋植型与非埋植型种植体在义齿修复受载后,种植体-骨界面中骨涎蛋白表达的变化。方法:选用纯系Beagle犬8只,在其下颌左右两侧分别植入埋植型与非埋植型种植体各3枚,每侧第一枚不作义齿修复,用于对照,其余各枚均采用固定金属全冠行种植义齿修复,分别于修复后2、4、8、12周各处死2只动物,取下颌骨相应骨段,于每个种植体周围10 mm处制作颊舌向纵行石蜡切片(片厚4μm),采用免疫组化染色方法检测种植体-骨界面骨涎蛋白表达情况。结果:两对照组(未修复组)种植体-骨界面组织中骨涎蛋白均呈弱阳性表达,且各时间点表达均无明显差异(P>0.05)。而两实验组(修复受载后)种植体-骨界面组织中骨涎蛋白表达的阳性强度均随观察时间延长而增加,8周时达高峰,12周时出现回落;同一时间点内埋植型与非埋植型种植体相比,无论是对照组还是实验组,各时间点内的骨涎蛋白表达程度均无显著性差异(P>0.05)。结论:埋植型和非埋植型种植体在义齿修复受载后,所承受的机械负荷均能刺激种植体-骨界面骨涎蛋白表达发生规律性变化,促进其界面的改建;且埋植型和非埋植型种植义齿受载后其界面的改建之间无显著性差异(P>0.05)。

AIM ： To compare the expression of bone sialoprotein （BSP） in different implant-bone interface after loading of submerged and nonsubmerged implant dentures. METHODS：Eight adult inbred beagle dogs were used to build the animal model. 3 submerged and 3 nonsubmerged implants were implanted into each side of the mandible respectively. The first implant at each side served as control and no denture was made. The remaining implants were fixed by metal full crown. 2, 4, 8, and 12 weeks after crown restoration, 2 dogs were sacrificed respectively. The cor- responding mandible bone segments were taken. Buccolingual longitudinal paraffin sections, about 10 mm to each im- plant ,were made （the thickness of each piece was 4 p,m）. Immunohistochemistry was used to observe the dynamic ex- pression of bone sialoprotein in the implant-bone interface. RESULTS： In the two control groups （without loading）, weak expression of BSP in the implant-bone interface was observed. The expression level was not significantly changed at different time points. In the two experimental groups （with loading）, strong BSP expression in the implant-bone interface was observed and BSP expression enhanced with loading time. Peak expression was at 8-week and afterwards, BSP expression decreased and returned to the level of the control group at 12-week. No obvious difference of BSP expression in the implant-bone interface was observed between the submerged and nonsubmerged implants at the same time points. CONCLUSION： The mechanical loads from the submerged and nonsubmerged implant denture both can stimulate the expression of BSP in the implant-bone interface, promoting the interface remodeling. Furthermore, there is no distinct difference in the implant-bone interface remodeling after loading of submerged and nonsubmerged implants.