胰腺癌细胞Panc-1中AKT2基因表达对吉西他滨敏感性的影响

Effects of AKT2 expression on gemcitabine sensitivity in pancreatic cancer cell line Panc-1

目的探讨胰腺癌细胞Panc-1中AKT2基因表达对吉西他滨敏感性的影响。方法体外将AKT2特异性shRNA表达载体pAKT2-shRNA转染胰癌细胞株Panc-1。应用RT—PCR、Westernblot检测转染shRNA后Pane-1细胞AKT2基因和蛋白的表达变化，应用MTT法检测干扰AKT2后Pane-1细胞对吉西他滨敏感性的变化。结果转染pAKT2～shRNA后Pane-1细胞AKT2基因和蛋白的表达水平明显下降；吉西他滨对Pane-1细胞的半数抑制量从（1．96±0．22）mg／L降到（O．24±0．03）mg／L，Panc-1细胞对吉西他滨的敏感性明显增加。结论用pAKT2-shRNA抑制AKT2基因表达，能增加人胰癌细胞株Panc-1对吉西他滨的敏感性。

Objective To explore the effects of AKT2 expression in pancreatic cancer cell line Panc-1 on the sensitivity towards to gemcitabine. Methods The expression vector pAKT2-shRNA was constructed and transfected into Panc-1 cells by lipofecton. The mRNA and protein expression levels of AKT2 were detected by RT-PCR and Western blot assays, respectively. The changes of gem- citabine sensitivity after shRNA were examined by the MTT assay. Results The mRNA and protein levels of AKT2 in Panc-1 cells were significantly decreased after the transfection, and the median inhi- bition concentration of gemcitabine against Panc-1 cells was reduced from （1.96±0.22） mg/L to （0.24±0.03） mg/L. The sensitivity of Panc-1 cells to gemcitabine was increased significantly after pAKT2-shRNA transfeetion. Conclusion The sensitivity of Panc-1 cells to gemcitabine could be en- hanced by pAKT2-shRNA.