溃疡性结肠炎患者外周血IL-22及相关CD4^＋T细胞亚群的表达

Expression of interleukin-22 and relative CD4^＋ T cell subsets in ulcerative colitis

目的通过检测溃疡性结肠炎（UC）患者外周血中IL-22及相关CD4^＋T细胞亚群的表达，探讨其在UC发病机制中的可能作用。方法以我院住院治疗的35例UC患者及35例健康对照者为研究对象，应用ELISA法检测外周血血浆中IL-22的含量，采用流式细胞术检测Th1、Th17、Th22细胞亚群的比例，并分析其与疾病活动度的关系。结果UC组患者血浆中IL-22的含量为（354．12±104．22）pg／ml，显著高于对照组（P〈0．05），其中重度患者增高明显；UC组患者外周血Th17细胞比例[（2．36±0．94）％]显著高于对照组（P〈0．05），其中，中、重度患者增高明显；UC组患者外周血Th22细胞比例[（2．27±0．87）％]显著高于对照组（P〈0．05），其中，重度患者增高明显；而UC组患者Th1细胞比例与对照组比较无明显差异。结论UC患者外周血中IL-22水平、Th17、Th22细胞比例明显升高，且与疾病活动度密切相关。

Objective To detect the expression of interleukin-22 （IL-22） and relative CD4^＋ T cell subsets in patients with ulcerative colitis （ UC ）, and to explore their roles in the pathogenesis of UC. Methods Thirty-five adult UC patients were enrolled in this study and 35 healthy subjects were taken as control. Plasma IL-22 level was quantified by ELISA. The percentages of Th1, Th17 and Th22 cells in peripheral blood were determined by flow cytometry. The relationships of these results and disease activity were analyzed. Results Plasma IL-22 levels in UC patients [ （354. 12 ±104.22） pg/ml ] were significantly higher than that of healthy controls （P〈0.05）, and the levels increased significantly in severely active patients. The percentages of Th17 cells in UC patients [ （2.36±0.94）% ] were elevated compared to healthy controls （P〈0.05）, and the percentages increased significantly in moderately active and severely active patients. The percentages of Th22 ceils in UC patients [ （2.27±0.87）% ] were elevated compared to healthy controls （P〈0.05）, and the percentages increased significantly in severely active patients. The percentage of Th1 cells was not significantly different between UC patients and normal controls. Conclusion Our resuits demonstrate elevated IL-22 correlated to Th17 and Th22 cells may play an important role in the immunopathogenesis of UC.