HLA—G 14 bp基因多态性与儿童Epstein—Barr病毒感染的相关性分析

HLA-G 14 bp gene polymorphism in relation to Epstein-Barr virus infection in children

目的通过检测Epstein-Barr病毒（EBV）感染传染性单核细胞增多症（IM）患儿人类白细胞抗原G（HLA-G）14bp插入／缺失多态性及血浆可溶性HLA—G（sHLA—G）水平，探讨其与儿童EBV感染IM的相关性。方法采用PCR结合聚丙烯酰胺凝胶电泳（PCR—PAGE）技术，对102例EBV感染IM患儿及165例正常对照儿童进行了HLA—G14bp插入／缺失多态性检测；采用ELISA检测了51例EBV感染IM患儿及146例正常对照儿童血浆sHLA-G水平。结果EBV感染IM组与正常对照组HLA-G14bp插入／缺失基因型多态性分布频率差异有统计学意义（χ^2=6．742，P=0．034），两组等位基因分布频率差异亦有统计学意义（χ^2=6．672，P=0．01）；EBV感染IM组血浆sHLA-G水平明显高于正常对照组，经检验差异有统计学意义（Z=-9．472，P〈0．01），EBV感染IM组不同基因型间血浆sHLA—G水平差异有统计学意义（H=6．09，P=0．048），14bp^＋/＋基因型组血浆sHLA-G水平明显低于另两组基因型（Z=-2．376，P=0．018）。结论HLA-G14bp插入／缺失基因型多态性与儿童EBV感染IM的发生有关，携带14bp^-/-基因型或缺失14bp等位基因的儿童可能更易发生EBV感染。血浆sHLA—G水平可作为EBV感染IM的辅助诊断指标之一。

Objective To explore the relationship between the HLA-G 14 bp insertion/deletion polymorphism and the infection of Epstein-Barr virus（EBV） for children. Methods The study genotyped HLA-G 14 bp insertion/deletion polymorphism of 102 infectious mononucleosis children and 165 normal controls by PCR-PAGE, detected the plasma sHLA-G level of 51 infectious mononucleosis children and 146 normal controls by ELISA. Results A significant difference was observed for the frequencies of the HLA-G 14 bp genotype between the two groups （χ^2 = 6.742, P = 0.034）, and a significant difference was also observed for the 14 bp allele frequencies between the two groups（χ^2= 6.672, P= 0.01 ）. The plasma s HLA-G levels in the infectious mononucleosis children were dramatically higher than that in normal controls, and a significant difference was observed between the two groups（Z=-9. 472, P〈0.01 ）. Among the infectious mononucleosis children, levels of sHLA-G was find a significant difference between the three genotypes of HLA-G 14 bp insertion/deletion polymorphism （H = 6.09,P = 0.048）, and the level of sHLA-G with 14 bp^＋/＋ genotype was markedly lower than that of the two other genotypes （Z =-2. 376, P= 0. 018）. Conclusion There was a relationship between the HLA-G 14 bp insertion/deletion polymorphism and the susceptibility to the infectious mononucleosis for children. Children who carried the 14 bp^-/- genotype or deleted the 14 bp allele may have a significantly increased risk of the infection of EBV. The plasma sHLA-G might be considered as an index for auxiliary diagnosis infectious mononucleosis.