青蒿素对狼疮性肾炎小鼠外周血单个核细胞GRα mRNA、GRβ mRNA及肾组织P300/CBP蛋白表达的影响

Effect of Artemisinin on the Expressions of GRα mRNA,GRβ mRNA and P300/CBP Protein in Lupus Nephritis Mice

目的:探讨青蒿素对狼疮性肾炎小鼠外周血单个核细胞GRαmRNA、GRβmRNA及肾组织P300/CBP蛋白表达的影响。方法:40只雌性B6D2F1代杂交小鼠随机分为正常对照组、模型组、泼尼松组(泼尼松混悬液灌养:6.45 mg/kg)及青蒿素组(青蒿素混悬液灌养:150 mg/kg)。通过诱导方法建立狼疮性肾炎小鼠模型,分别采用RT-PCR及免疫组化(SP)法等检测技术测定外周血单个核细胞GRα mRNA、GRβ mRNA表达及肾组织转录共激活因子P300/CBP蛋白表达情况,观察青蒿素对上述指标的影响。结果:与模型组比较,青蒿素组动物外周血单个核细胞GRαmRNA和肾组织转录共激活因子P300/CBP蛋白表达均有不同程度升高(P<0.01),GRβ mRNA表达显著降低(P<0.05或P<0.01),且青蒿素对GR αmRNA和转录共激活因子P300/CBP蛋白表达的影响明显优于泼尼松组(P<0.01)。结论:青蒿素治疗狼疮性肾炎的作用机制可能与调节外周血单个核细胞GRα mRNA、GRβ mR-NA及肾组织P300/CBP蛋白表达有关。

Objective ：To investigate the effects of Artemisinin on the expressions of GRα mRNA, GRβ mRNA and P300/CBP pro- tein in lupus nephritis mice. Methods：Forty hybrid female mice were randomly and equally divided into four groups with the method of random number table： control group, model group, prednisone group administrated with 6.45 mg/kg Artemisinin （Art） suspension. A mice model of LN was established by injection with living lymph cell suspension. The expressions of GC receptor a （GRot） mRNA, GC receptor β （GRβ） mRNA in peripheral blood mononuclear cells （PBMCs）, and transcriptional coactivator P300/CBP protein in renal tissue were respectively measured by the technique of RT - PCR and immunohistochemical assay. Results： Compared with the model group, the expression of transcriptional coaetivator P300/CBP protein in renal tissue and GRα mRNA in PBMCs of treatment groups was increased significantly, GRβ mRNA expression was significantly decreased （ P 〈 0.05 or P 〈 0.01 ）. And the Art group had a better effect on the expression of GRα mRNA and transcriptional coactivator P300/CBP protein than that of the prednisone group （P 〈 0.01 ）. Conclusion ： The underlying therapeutic mechanism may be correlated with the regulation of Art on the expressions of GRα mRNA, GRβ mRNA in PBMC and transcriptional coactivator P300/CBP protein in renal tissue.