新型微流控多剪切力芯片的数值模拟及其应用(英文)

Computational Simulation and Application for a Novel Microfluidic Multishear Device

设计了一个能同时产生多个剪切力的微流控芯片设备,并对其剪切力的分布进行了数值模拟.利用了计算机流体动力学软件FLUTNT描述了流速和剪切力的关系.模拟的结果显示,剪切力是正比于流速的;细胞培养小室的中心区域存在一个均一的剪切力区域,该区域的大小依赖于培养小室的宽度大小;剪切力的值在较窄的小室,如400和800μm宽的小室出现了偏离.最后,该微流控设备被应用在观察流体剪切力对成骨细胞的钙信号影响的实验上.

A microfluidic multishear device used for quantitatively measuring cell behaviors was designed and computationatly simulated on the distribution of shear stress. The relationship between flow rate and shear stress was characterized using the Computational Fluid Dynamics （CFD）. Simulation results showed that wall shear stress was proportional to flow rate. There was a uniform shear stress area in the central region of cell culture chamber. This area size varied depending on the chamber width. The shear stress value showed a deviation at a narrow chamber, such as 400 and 800 μm wide chambers. Finally, an application on observing calcium response under shear stress in osteoblasts was shown.