灯盏花种子发芽标准化研究

Study on Seed Germination Testing Standardization ofErigeron breviscapus(Vant.)Hand.-Mazz.

目的:确立灯盏花种子发芽最适条件。方法:试验设6种不同浓度的NaClO和KMnO4以及无菌水和自来水(简称为CK为对照)共14个处理进行抑菌试验;设5、10、15、20、25、30、35℃恒温及35/25、30/20、25/15、20/10、15/6℃变温共12种温度处理,设纸上、纸间、砂床、土壤、珍珠岩共5种发芽床处理,光照设黑暗和光照2个处理。结果:灯盏花种子发芽的适宜条件为发芽前先用清水纸间浸种4h,然后用1%NaClO预处理30min,最后将种子置于纸床上,于25℃恒温光照条件下进行发芽,初次计数时间为发芽后的第7天,末次计数时间为发芽后的第12天。结论:试验确立的灯盏花种子发芽最适条件可为灯盏花种子质量检验规程的制定提供依据。

To screen the optimal germination condition of Erigeron breviscapus （Vant.）Hand. -Mazz. , a total of 14 kinds of disinfection liquid treatments were used including 6 concentrations of NaC10 and KMnO4 solutions, respectively, sterile water and ta Pwater（ for short CK）. 12 kinds of temperature treatments were conducted including seven constant temperatures,5,10,15,20,25,30, and 35 °C, and five variable temperatures,35/25 °C ,30/20 °C, 25/ 15 °C ,20/10 °C, and 15/6 °C. Five kinds of germination beds, including top of papers ,between papers, sand bed, soil, and pearl rock, were adopted. The dark and light conditions were set. The results showed that the optimal seed germination conditions of E. breviscapus were as followings ： the seeds were soaked for 4 h with, then sterilized for 30 min in 1% NaC10 solution, and finally transferred to a filter paper at 25 °C for germination. The initial counting was firstly conducted at 7th d after germination, and the last were at 12 d. The optimal seed germination conditions were ascertained and could be referred as quality test standards, and thus provide a basis for the development of the inspection rules of seed quality control in E. breviscaous.