不同预处理对长寿沙田柚根尖染色体制片的影响

Effects of Pretreatment on Chromosome Squashing of Root Tip of Citrus grandis Osbeck cv.Shatianyou Changshou

本研究采用0.1%秋水仙素、对二氯苯饱和水溶液、对二氯苯-α-溴萘混合液这3种常用于植物染色体预处理的药剂,研究了不同处理方式对长寿沙田柚根尖细胞染色体制片效果的影响。结果表明:利用对二氯苯-α-溴萘混合液预处理根尖12h效果最好,中期分裂指数高,染色体分散、清晰。

With materials of the root tips of Citrus grandis Osbeck cv. Shatianyou Changshou, the effects of three pretreatments on chromosome squashing were investigated, which are 0.1% colchicine, saturated-p-dichlorobenzene and p-dichlorobenzenc -α-bromonaphthalene mixed solution. The results showed that the best pretreatment was treating with p-dichlorobenzene-α-bromonaphthalene mixed solution for 12 hours, the index of cell division at metaphase being highest, also the chromosomes dispersed well and clear form.