羊踯躅嫩叶离体培养和植株高效再生技术研究

Study on technology of in vitroculture and efficient regeneration of plantlet with the new leaves of Rhododendron molle(Blume) G.Don

【目的】建立羊踯躅嫩叶离体培养及植株高效再生体系。【方法】以羊踯躅新生嫩叶为外植体,筛选嫩叶愈伤组织诱导、再分化及生根培养基,并以再生植株茎节为试材,建立高效植株再生体系。【结果】最适合羊踯躅嫩叶愈伤组织诱导的培养基为1/2DR+2.40mg/L 2ip+0.80mg/L NAA,诱导率为99.5%;愈伤组织再分化的最佳培养基为1/2DR+2.90mg/L 2ip+0.01mg/L NAA+1.75mg/L KT,分化率为99.7%;适宜生根的培养基为1/4DR+0.06mg/L ZT+0.02mg/L NAA,生根率达99.0%。利用再生植株茎节的快繁结果表明,在28d的培养周期内,每节段平均增殖达5倍以上。【结论】成功建立了羊踯躅嫩叶愈伤组织诱导、再分化芽苗和植株高效再生体系,可以满足羊踯躅工厂化育苗的需要。

【Objective】 The study was to establish in vitro culture and efficient regeneration of plantlet system of Rhododendron molle（Blume） G.Don.【Method】 The new leaves of Rhododendron molle（Blume） G.Don were used as explants for the experiment.Screening mediums of callus induction,callus redifferentiation and rooting,and stems with nodes were taken as the experiment materials to establish efficient regeneration system of plantlet.【Result】 The results showed that 1/2 DR＋2.40 mg/L 2ip＋0.80 mg/L NAA was the best suit for callus from new leaves,the rate of callus was 99.5%;1/2 DR＋2.90 mg/L 2ip＋0.01 mg/L NAA＋1.75 mg/L KT for shoots redifferentiation,and the rate of differentiation was 99.7%;1/4 DR＋0.06 mg/L ZT＋0.02 mg/L NAA for rooting,the rate of rooting was more than 99.0%;Each stem with one node was cut from regenerated shoots and cultured for propagation,and a 5-fold proliferation rate was achieved within 28 days.【Conclusion】 In this study,callus induction,shoots redifferentiation with the new leaves of Rhododendron molle（Blume） G.Don and efficient regeneration system of plantlet have been established,which could satisfy the need for industralized seedlings of Rhododendron molle（Blume） G.Don.