摘要
目的建立高效液相色谱测定人血清中氯氮平(抗精神病药)浓度的方法。方法血清样品上SPE柱,甲醇为洗脱剂,65℃氮气吹干后复溶进样测定。用Hypersil C18色谱柱,流动相为甲醇-水=75∶25(加入0.3%三乙胺,用冰醋酸调节pH=8.0),流速为0.8 mL.min-1,检测波长243 nm。结果氯氮平血清样品线性范围为0.05~10.00 mg.L-1,低、中、高3种不同浓度(0.22,1.08,3.22 mg.L-1)方法回收率分别为102.0%,99.7%,99.4%,日内、日间RSD均小于5%。结论本方法准确性好,灵敏度高,操作简便,可以满足氯氮平血药浓度监测和药物中毒分析的要求。
Objective To establish a HPLC method for determination of the concentration of clozapine in serum.Methods The analysis was conducted with Hypersil C18 column.Methanol-water(75∶25)(0.3% triethylamine was added and glacial acetic acid was used to regulate pH to 8.0) was served as mobile phase,the flow number was 0.8 mL·min-1,and the detecting wavelengh was at 243 nm.Serum sample was applied to SPE column,and the eluting agent was methanol.The eluant was collected and was dried in 65 ℃ with Nitrogen gas.Results The linear range of clozapine in serum was 0.05-10.00 mg·L-1.The recovery rate of low concentration,moderate concentration and high concentration(0.22,1.08,3.22 mg·L-1) was 102.0%,99.7%,99.4% respectively.Both of the intra-day and inter-day RSD were less than 5%.Conclusion This method is accurate,sensitive,and simple.It is suitable for the determination of the concentration of clozapine in serum and the analysis of drug poisoning of clozapine.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2012年第6期456-457,461,共3页
The Chinese Journal of Clinical Pharmacology