SPF鸡盲肠复合微生态制剂代替抗生素预防肉仔鸡沙门菌感染

Prevention of Salmonella infection in broilers with SPF chicken cecal probiotics complex instead of antibiotics

目的在雏鸡盲肠尽快建立完整健康菌群,通过"竞争排除(Competitive exclusion,CE)"抑制病原菌定植感染。方法提取SPF鸡盲肠菌群,排除沙门菌感染的可能,制成复合微生态制剂,在鸡胚孵化19 d啄壳时和21 d雏鸡全出壳时进行两次喷雾接种,监测鸡群沙门菌抗体和粪便中沙门菌,测定成活率、生产性能。结果进行喷雾接种后的小鸡至12日龄可以完全不使用抗生素,成活率、采食量与体重略高于使用抗生素的对照组,能提高饲料报酬,饲养12、24、35 d均未检出粪便沙门菌和血液沙门菌抗体,全程无沙门菌感染。结论 SPF鸡盲肠复合微生态制剂能代替抗生素预防肉仔鸡沙门菌感染。

Objective To establish complex and complete cecal flora chickens to prevent the colonization and infection of pathogens by competitive exclusion （CE）. Method Complex microeeological preparation was extracted from caecum flora of SPF chickens which were excluded from the possibility of Salmonella infection. The preparation was sprayed on eggs at 19 days （ when they pecking the shells） and 21 days （ when they pecking fully out of shells） of hatching, respectively. Salmonella in the excrement or its antibodies in the blood were monitored and the survival rate and production performance were recorded and analyzed. Result The inoculated chicken no longer needed antibiotics up to 12 days after birth; Their survival rate, weight and feed intake were slightly higher compared with the control group. A better feed reward could be observed. No Sal- monella in the excrement or its antibodies in the blood could be observed after the chickens were fed for 12, 24 or 35 days. There was no Salmonella infection during the growth of these chickens. Conclusion Salmonella infection in broilers can be prevented with SPF chicken cecal probiOtics complex instead of antibiotics.