正辛胺改性海藻酸钠凝胶微球的制备及其性质研究

Study on the Preparation and Properties of Sodium Alginate Gel Microspheres Modified by n-octyl Amine

目的:制备正辛胺改性海藻酸钠凝胶微球,并研究其性质。方法:以超声波辅助氧化法制备多醛基海藻酸钠,通过希夫碱反应制备正辛胺改性海藻酸钠,并表征其结构;以乳化-内部凝胶化技术制备负载小分子抗肿瘤药物β-榄香烯的改性海藻酸钠凝胶微球,采用气相色谱法测定其8、15、24、48h时的累积释放率及海藻酸钠和正辛胺改性海藻酸钠凝胶微球中β-榄香烯的包封率。结果:表征并证实了多醛基海藻酸钠和正辛胺改性海藻酸钠的结构;制备得到的改性海藻酸钠凝胶微球中8、15、24、48h时β-榄香烯的累积释放率分别为16%、28%、40%、83%;海藻酸钠和正辛胺改性海藻酸钠凝胶微球中β-榄香烯的包封率分别为36%、73%。结论:制备的正辛胺改性海藻酸钠凝胶微球,具有优良的缓释性能,对β-榄香烯的包封率高。

OBJECTIVE： To prepare Sodium alginate gel microspheres modified by n-octyl amine, and to investigate the prop- erty of it. METHODS ： Multialdehyde sodium alginate was prepared by oxidation with aid of ultrasound, Sodium alginate modified by n-octyl amine was prepared through schiff base reaction and the structure of them were characterized. Modified sodium alginate gel microspheres of β-elemene were prepared by emulsion-internal gelatination technology. Accumulative drug release rate of β-el- emene in microspheres were determined by GC at 8, 15, 24, 48 h, and encapsulation coefficency ofβ-elemene in sodium alginate and Sodium alginate gel microspheres modified by n-octyl amine were also determined. RESULTS： The structures of multialdehyde sodium alginate and Sodium alginate gel microspheres modified by n-octyl amine were characterized and confirmed. Accumulative drug release rates ofβ-elemene in prepared microspheres were 16%, 28%, 40%, 83% at 8, 15, 24, 48 h. Encapsulation coeffi- cencies of β-elemene in sodium alginate or Sodium alginate gel microspheres modified by n-octyl amine were 36% and 73%, re- spectively. CONCLUSION： Modified microspheres are stable and have good sustained drug release, and have higher encapsulation coefficency for B-elemene.