三种致病性细菌的SERS光谱研究

SERS Spectroscopy Study of Three Pathogenic Bacteria

用便携式拉曼光谱仪获得了金黄色葡萄球菌、变形杆菌、大肠杆菌在微波法制备的纳米银溶胶上的表面增强拉曼光谱。金黄色葡萄球菌在725,1 330,1 450cm-1有明显的拉曼振动峰,变形杆菌在650,725,950,1 325,1 463cm-1有明显的拉曼振动峰,大肠杆菌在650,950,1 125,1 242,1 320,1 457cm-1有明显的拉曼振动峰,对各个峰进行了初步归属。三种细菌的拉曼振动峰的位置和强度区别明显,因此SERS技术可以用于大肠杆菌、金黄色葡萄球菌和变形杆菌的快速鉴别。

The SERS spectra of staphylococcus aureus, proteus, and Escherichia coli was obtained on colloidal Ag nanoparticles prepared by the microwave method with the portable Raman spectrometer. Staphylococcus aureus have obvious Raman vibrating peak at 725, 1 330 and 1 450 cm-l , proteus have obvious Raman vibrating peaks at 650, 725, 950, 1 325 and 1 463 cm-I , while E. coli have obvious Raman vibrating peaks at 650, 950, 1 125, 1 242,1 320 and 1 457 cm 1. Each peak was assigned prelimi- narily. Not only the position of Raman vibration peaks but also the intensity of the three bacteria is obviously different, so SERS can be used for identification and distinction of E. coli, staphylococcus aureus and proteus.