湿法消解-火焰原子吸收法测定动物样品中六种金属元素

Determination of Six Metal Elements in Animal Samples by Flame Atomic Absorption Spectrometry with Wet Digestion

使用硝酸-高氯酸湿法消解火焰原子吸收法测定大、小鼠的血清、粪便、尿液和不同组织器官(心、肾、胃、肝、脾、肠和肺)中Ca,Mg,K,Na,Cu,Fe等六种金属元素。以硝酸与高氯酸的混合液(4∶1,φ)为消解液,加热条件为120℃,标准曲线相关系数为0.999 4～0.999 8,相对标准偏差(RSD)在0.33%～4.52%(n=6)之间,加样回收率均在97.7%～104.2%之间,满足生物样品中各元素含量测定的要求。本研究建立的动物样品中六种金属元素含量的测定方法具有操作简便快捷、灵敏度高、所需样品量少、结果准确可靠等优点,可广泛用于生物样品中多种金属元素的测定。

The contents of 6 metal elements （Ca, Mg, K, Na, Cu and Fe ） were determined in serum, urine, feces and different tissue and organs （heart, kidney, stomach, liver, spleen, intestine and lung） of Wistar rats and mice by flame atomic absorption spectrometry （FAAS） with wet digestion. The samples were digested by the mixture of HNO3 and HCIO4 （4 ： 1, V/V） at 120 ℃, the correlation coefficient for the standard curves was 0. 999 4-0. 999 8, the relative standard deviation （RSD） was from 0, 33% to 4. 52%, and the recovery for the samples was from 97. 7% to 104.2%, meeting the demand of elements content deter- mination in biological samples. The assay method for the determination of the 6 metal elements in animal samples established in this study has the advantages of easy operation, high sensitivity, less sample requiring and accurate results, and the method can be widely used in the determination of various metal elements in biological samples.