番茄材料“0946”和“0949”再生体系的研究

Study on Regenereration System of Tomato Accessions ‘0946’ and ‘0949’

以番茄材料‘0946’、‘0949’为试材,用MS做基本培养基,研究不同外植体、不同激素组合对愈伤组织诱导、芽体分化、生根情况的影响,以建立一套适合的番茄再生体系。结果表明:诱导愈伤组织及不定芽分化的最佳培养基为2.0 mg/L 6-BA+(0.1～0.2)mg/L IAA;以0.2 mg/L IAA诱生生根效果最好。该研究不仅针对该材料建立了番茄再生体系,同时也为利用番茄进行遗传转化奠定了技术基础。

Tomato accessions’0946’,’0949’ were used as test materials for establishing tomato veyeneration system,MS media was used as basic media,calli induction,shoot differentiation and rooting condition of different explants and different hormone combinations were investigated.The results showed that MS＋2.0 mg/L 6-BA＋0.1～0.2 mg/L IAA was the optimal medium for calli induction and adventitious bud differentiation,MS＋0.2 mg/L IAA was the optimal medium for rooting.This study not only establish the regeneration system for tomato accessions ’ 0946’ and’ 0949’,but also provide technological basis for tomato genetic transformation.