培养基种类、BA浓度和蔗糖浓度对春石斛组培苗增殖的影响

Effect of Several External Factors on Proliferation and Growth of Dendrobium casi flake

以春石斛(Dendrobium casiflake)的组培苗为试材,研究培养基的种类、BA浓度、蔗糖浓度等因素对不定芽增殖生长的影响,为春石斛种苗的规模化生产提供理论依据。结果表明:在MS培养基中不定芽分化及生长能力明显好于White、VW、SH、B_5及KC培养基,培养30 d后每个外植体平均分化出3.7个健壮的苗;在MS培养基中加入BA 3.0 mg/L以及蔗糖30 g/L时,春石斛不定芽增殖数多,且生长良好。

With adventitious shoots proliferated through tissue culture of D.casi flake as experimental material,the effect of several factors such as the medium types,the concentrations of BA,the concentrations of sucrose etc on the proliferation and growth of D.casiflake were studied.To provide the theoretical basis for scale production of D.casiflake seedling.The results showed that the effect of MS medium on the proliferation and growth of D.casiflake was batter than other kinds of medium,and should help improve the differentiation of adventitious shoots.It had obvious promoting effect on the differentiation of adventitious shoots,fresh weight and dry weight when BA at 3.0 mg/L and sucrose at 30 g/L were added into MS medium which were perfect for the proliferation and growth of D.casiflake.