千层金组培快繁技术研究

Study on the Tissue Culture and Rapid Propagation Technology for Melaleuca bracteata

以嫩茎为外殖体,以MS为基本培养基,分别加入不同浓度6-BA、NAA、IBA,研究不同浓度激素及组合对千层金组培快繁的影响。结果表明:诱导芽的最佳培养基为MS+6-BA2.0 mg/L+NAA 0.2 mg/L,诱导率达63.6%;继代培养最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,增殖系数达6.3,且芽苗健壮;壮苗培养的最佳培养基为MS+NAA 0.1 mg/L,每瓶产苗数达16.2株;最佳生根培养基为1/2MS+NAA 0.1 mg/L,21 d生根率达100%,平均根数达9.6条。采用泥炭土与椰糠体积各半混合基质进行移栽,30 d成活率达95.7%,能够满足工厂化苗木生产的要求。

With employing tender bine as explant,MS as basic medium mixing different density 6-BA,NAA,IBA,effect of different concentration hormone and its combination on tissue culture and rapid propagation of Melaleuca bracteata were studied.The results indicated that the optimal medium of inducing sprout was MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L, and the induction rate achieved 63.6%;the optimal medium of successive proliferation sprout was MS＋6-BA 1.0 mg/L＋ NAA 0.2 mg/L,the proliferation coefficient achieved 6.3 with sprout in good health;the optimal medium of seedling cultivation was MS＋NAA 0.1 mg/L,and the number of seedling per training bottle achieved 16.2;the optimal medium of root training was 1/2MS＋NAA 0.1 mg/L,the rooting rate in 21 d achieved 100%,and the mean number of roots achieved 9.6.Using peat soil mixing coconut chaff half in half in volume for transplanting,the survival rate in 30 d achieved 95.7%,it could fit factory seedlings production requirements.