摘要
利用热激的β-乳球蛋白(β-Lg)与矢车菊素-3-葡萄糖苷(C3G)共组装形成纳米粒以保护C3G的抗氧化活性。溶液pH值为2.5~6.5、蛋白热激温度为30~85℃及β-Lg与C3G不同摩尔比例(1∶2~1∶32)对纳米体系的粒径、Zeta电位、包埋率及单位蛋白载药量具有显著影响。在pH 6.5时能够形成稳定的纳米分散体,在热激温度85℃、β-Lg与C3G摩尔比为1∶2时,β-Lg对C3G的抗氧化活性具有最好的保护作用。
Cyanidin-3-glucoside (C3G) was coated by heat treatment with β-1actoglobulin (β-Lg) for the pres-ervation of antioxidant activity. The effects of pH (2.5-6.5) , heating temperature of β-Lg (30-85 ℃ ) and the molar ratio of β-Lg to C3G (1: 2-1: 32) on the properties of β-Lg-C3G complexes were studied. All three factors signifi-cantly influenced the particle size, zeta-potential, entrapment efficiency of C3G and C3G encapsulation with β-Lg particles. Stable and clear solution could be obtained at pH 6.5. The highest protection of EGCG antioxidant activity was obtained with β-Lg heated at 85 ℃ and the molar ratio of 1:2 (β-Lg: C3G).
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2012年第5期32-36,共5页
Food and Fermentation Industries
基金
教育部博士点基金项目(0113326110001)
浙江省自然科学基金重点项目(Z12C160014)
国家科技支撑计划课题(2011BAD01B03-5)