活性污泥中1株产PHB丝状细菌的分离与鉴定

Isolation and Characterization of Filamentous Bacteria HY10 Accumulating Poly-β-hydroxybutyrate ( PHB) from Activated Sludge

从污水处理厂等活性污泥样品中分离到了19株丝状细菌,通过苏丹黑染色法和紫外吸收光谱扫描技术证明这些丝状细菌能够在菌体内积累聚β-羟基丁酸(PHB)。采用氯仿乙醇法提取PHB,并采用紫外吸收法进行定量测定,筛选出1株高产PHB丝状细菌HY10,细胞PHB含量为(37.24±0.74)%,PHB产量为1.15±0.06 g/L。综合该菌株的菌落形态和16S rDNA序列(GenBank No.JQ353768)分析结果将HY10菌株鉴定为假蕈状芽孢杆菌(Bacillus pseudomycoides)。

19 strains of filamentous bacteria that accumulated poly-β-hydroxybutyrate （PHB） were isolated from activated sludge by enrichment cultivation. Staining with Sudan Black B was applied to detect PHAs. Quantitative es-timation of PHB extracted from the isolates was done by Ultraviolet spectrophotometer method. PHB was extracted from these strains through the CHC13 - CH3CH32OH method. The PHA productivities of strain HY10 was the highest in 19 isolates mentioned above, in which PHB yield and productivities were（37.24 ± 0.74）% dry cell weight （DCW） and 1.15 ±0.06 g/L, respectively when grown in a medium containing 20 g/L sucrose. According to its clone mor-phological and the 16S rDNA sequence （ GenBank No. JQ353768）, it suggested that strain HY10 was Bacillus pseud-omycoides.