快速生化需氧量微生物传感器的应用

Application of Rapid Biochemical Oxygen Demand Biosensor

采用混合菌BODseed制备微生物传感器快速监测生化需氧量(BOD),研究了矫正溶液选择、样品自降解及恒温装置对测量准确度的影响。结果表明,采用葡萄糖-谷氨酸矫正溶液测得污水处理厂污水结果较BOD5方法偏低50%以上,而以污水本身制作矫正曲线取得了与BOD5一致的测试结果;样品在保存6 h后有机物自降解会使测量结果偏低40%;快速BOD在线监测时不仅需要对微生物传感器恒温,同时也需要对样品及缓冲溶液恒温,这有利于样品快速接近缓冲溶液温度,减少空气饱和时间,从而提高监测效率,提升测试准确度。

Multiple-species BODseed was used for fabricating biochemical oxygen demand （BOD） biosensors for rapid online BOD determination. Some factors to accuracy were studied such as calibration solution selection, sample self-biodegradation, and thermostatic device. Good results were obtained when wastewater was used as calibration solution instead of glucose and glutimic acid （GGA）. The self-biodegradation process made the rapid measurements inaccurate. Besides, thermostatic device used only for biosensors is defective, and it should be applied for maintaining a constant temperature of the samples and buffer, promoting the efficiency and accuracy of online monitoring.