地西他滨联合丙戊酸钠对白血病细胞株的促分化效应研究

Synergistic Effect of Decitabine and Valproic Acid on Induction of Differentiation in Leukemic HL-60 Cells

目的探讨地西他滨(decitabine,DCA)和丙戊酸钠(valproic acid,VPA)联用对白血病细胞株HL-60的促分化影响。方法设立分组如下:对照组,DCAA组(1.0 mol.L 1),DCA B组(4.0 mol.L 1),VPA组(2.0 mmol.L 1),联合用药A组(DCA1.0 mol.L 1+VPA 2.0 mmol.L 1),联合用药B组(DCA 4.0 mol.L 1+VPA 2.0 mmol.L 1),作用48 h。流式细胞术检测CD34、CD117 MFI以及CD11b、CD14表达率。结果联合用药A、B组的CD117和CD34 MFI显著低于其各自的单药组(P<0.01);联合用药A组的CD11b和CD14表达率和联合用药B组CD11b表达率显著低于其各自的单药组(P<0.01)。结论 VPA与DCA联用对HL 60细胞具促分化作用。

OBJECTIVE To investigate the synergistic effect of decitabine（DCA） and valproic acid（VPA） in differentiation induction in leukemic HL-60 cells. METHODS The drug groups were set as follows control group; DCA group A（1.0 μmol·L^-1）; DCA group B（4.0 μmol·L^-1）; VPA group（2.0 mmol·L^-1）; combination group A（DCA 1.0 μmol·L^-1＋VPA 2.0μmol·L^-1）; combination group B（DCA 4.0 μmol·L^-1＋VPA 2.0 mmol·L^-1）. The cells were treated for 48 h. The CD117, CD34 mean fluorescence intensity（MFI） and CD14, CDllb expression were detected by flow cytometry. RESULTS The CD117, CD34 MFI of combination group A and B were significantly lower than their corresponding concentration single drug group（P〈0.01）. Except CD14 of combination group B, the CD14, CDllb expressions of combination group A and B were significantly lower than their corresponding concentration single drug group（P〈0.01）. CONCLUSION There is an enhanced differentiation activity of combinations of DCA and VPA in HL-60 cells.