超声波复合酶法提取软紫草多糖的工艺优化

Optimization of Ultrasonic Pretreatment Followed by Enzymatic Hydrolysis for Extraction Technology of Polysaccharides from Arnebia Euchroma

目的研究超声波复合酶法提取软紫草多糖的最佳工艺条件。方法采用单因素分析和正交试验,以软紫草提取物中多糖得率和多糖含量为综合评价指标,考察超声波复合酶法中主要因素对软紫草多糖提取效果的影响。结果超声波提取优化工艺条件为:超声处理时间40 min,料液比1∶12,功率160 W;在超声波优化结果基础上,进一步进行超声波复合酶法处理,酶解最佳提取条件为酶解时间40 min,酶解温度55℃,pH 5.5,纤维素酶、果胶酶、蛋白酶添加量均为2%。结论超声波复合酶法双重处理可显著提高软紫草多糖的提取效果。

OBJECTIVE To study the optimal technological factors for extracting Arnebia euchroma polysaccharide with compound enzymes under ultrasonic. METHODS To develop an optimal procedure based on ultrasonic pretreatment followed by enzymatic hydrolysis for the extraction of polysaccharides from Arnebia euchroma, single factor and orthogonal design methods were used to deal with the effects of crucial ultrasonic pretreatment and hydrolysis parameters on polysaccharide yield. RESULTS The optimal values of ultrasonic power, pretreatment time and material/liquid ratio were determined to be 160 W, 40 min and 1 ： 12, respectively. The optimization of enzymatic hydrolysis was achieved through the combinatorial use of 2% cellulose, 2% pectinase and 2% neutral proteinase for the hydrolysis for 40 min at pH 5.5 and 55 ℃. CONCLUSION The combination of ultrasonic pretreatment and enzymatic hydrolysis may provide a good idea for the large-scale production of polysaccharides from Arnebia euchroma.