清胃黄连丸快速TLC鉴别与小檗碱新荧光扫描定量

Fast Identification by TLC and Quantitative Determination by Fluorescence TLCS for Qingwei Huanglian Pills

目的建立清胃黄连丸的快速TLC鉴别及小檗碱的高灵敏度荧光扫描定量方法。方法采用同一供试品溶液在2块薄层板上鉴别黄芩、牡丹皮、连翘、栀子和甘草,用荧光扫描法测定了清胃黄连丸中盐酸小檗碱的含量。结果盐酸小檗碱点样量在0.019 68～0.098 4 g内呈良好的线性关系。盐酸小檗碱平均回收率为99.41%,RSD为1.34%(n=9)。结论TLC鉴别斑点清晰,阴性无干扰。清胃黄连丸的快速TLC鉴别与小檗碱新荧光扫描定量,简便、快捷、准确、实用,有效提高了传统丸剂的质量检测速度。

OBJECTIVE To establish a method for fast identification by TLC and quantitative determination by fluorescence TLCS for Qingwei Huanglian pills. METHODS Scutellariae Radix, Moutan Coxtex, Forsythiae Fructus, Gardeniae Fructus, Glycyrrhizae Radix et Rhizoma were identified by TLC, using the same sample solution in two TLC plate. The content of berberine hydrochloride in Qingwei Huanglian pills was determined by fluorescence TLCS. RESULTS The methodological study showed a good linear correlation in the range from 0.019 68 to 0.098 4 μg of the berberine hydrochloride. The average recovery of berberine hydrochloride （n=9） was 99.41%, and RSD was 1.34%. CONCLUSION The method is simple, accurate, repeatable and with good precision. The method can be used for quality control of Qingwei Huanglian pills. The detection speed was effectively improved for assay of traditional pills.