超高效液相色谱法测定大鼠血浆中红景天苷含量

DETERMINATION OF SALIDROSIDE CONTENT IN RAT PLASMA BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY

目的建立红景天苷含量超高效液相色谱测定方法,并应用于红景天苷在大鼠体内的药代动力学研究。方法采用Waters ACQUITY UPLC H-CLASS T3色谱柱(50mm×2.1mm,1.8 m),流动相:乙腈/水(1/9),流速:0.25 ml/min,检测波长为280 nm,进样量5 l,内标为4-乙酰氨基酚。健康大鼠尾静脉注射给药后,在0.02、0.07、0.12、0.17、0.25、0.33、0.5、1.0、1.5、2 h时间点取血检测,并利用Kinetica软件对血药浓度-时间数据进行拟合。结果红景天苷质量浓度在1～400 g/ml范围内线性关系良好(r=0.9998),血浆中红景天苷的回收率≥90%,主要的药代动力学参数AUC(0-+∞)、T1/2、MRT、CL以及Vss分别为54.31±2.25 h/(mg/L)、0.65±0.04 h、0.77±0.03 h、0.37±0.02 L/h及0.28±0.02 L。结论本方法简单、快速、准确,适用于大鼠血浆中红景天苷的血药浓度测定及药代动力学研究。

Objective To establish ultraperfor mance liquid chromatography（UPLC） method for determination of salidroside content.Methods Waters ACQUITY UPLC H-CLASS T3 column（50mm×2.1mm,1.8um） was used,and the mobile phase consisted of acetonitrile/water（1/9） at the flow rate 0.25 ml/min,and the wavelength of UV detector was set at 280 nm with 5 μl sample injection volume,and 4-acetamidophenol was used as internal standard.The validated method was used to study the pharmacokinetic profile of salidroside in rat plasma after intravenous administration of salidroside through caudal vein.Blood samples were obtained at 0.02,0.07,0.12,0.17,0.25,0.33,0.5,1.0,1.5,2 h following intravenous administration and the data were processed with the software Kinetica.Results The calibration curve was linear（r=0.9998） over salidroside in rat plasma ranging from 1 to 400 mg/ml.The recovery was ≥90%.The primary pharmacokinetic parameters AUC（0-＋∞）,T1/2,MRT,CL and Vss were 54.31±2.25 h/（mg/L）,0.65±0.04 h,0.77±0.03 h,0.37±0.02 L/h and 0.28±0.02 L respectively.Conclusion The method is simple,rapid and suitable for determination of the concentration of salidroside in plasma and the study of pharmacokinetics.