猪伪狂犬病病毒SL株TK基因的克隆与生物信息学分析

Bioinformatics Analysis of TKGene of Pseudorabies Virus SL Strain from Pigs

本试验对伪狂犬病病毒SL株(四川分离株)TK基因进行克隆,并对TK基因的同源性、遗传进化树、密码子偏嗜性、氨基酸结构及亲疏水性、跨膜区、信号肽进行了分析。结果显示,本研究成功克隆了PRV SL株TK全基因,其编码区长963bp,编码320个氨基酸残基,与其他PRV分离株核苷酸序列同源性超过99.3%,编码的多肽链中疏水区域大于亲水区域,不含跨膜区及信号肽,不存在于病毒囊膜。

TK gene of pseudorabies virus SL strain was cloned. Based on the sequence, the homology, phylogeny tree, co- don bias, amino acid structure, hydrophilic and hydrophobicity, transmembrane region and signal peptide were analyzed. The results showed that TK gene was successfully cloned and it consisted 963 nucleotides encoding a protein of 320 amino acids. The TK gene shared over 99.3% homology with that of the other PRV strains. PRV TK was predicted to not have the trans- membrane region and signal peptide, and not be inside of virus envelope.