角蛋白26基因在超细型和细型细毛羊皮肤组织中的表达差异

Expression Difference of Keratin 26Gene of Skin Tissues in Superfine and Fine Merino Sheep

采用超细型和细型细毛羊皮肤组织为试验材料,以18SrRNA、β-actin、GAPDH等基因为内参,角蛋白26(keratin26,KRT26)为目的基因,建立了基于SYBR GreenⅠ染料技术的Real-time PCR检测体系,并分析KRT26基因在不同细度绵羊皮肤组织中的表达差异。结果表明,以cDNA为模板建立的标准曲线循环阈值(Ct)与标准cDNA模板在一定浓度范围内呈良好的线性关系,当以18SrRNA、β-actin、GAPDH作为内参基因时,KRT26基因在超细型细毛羊皮肤组织中的表达水平是细型细毛羊皮肤组织中的1.5倍。初步确定KRT26基因与毛细度具有相关性,这将为进一步研究分子育种和绵羊毛纤维细度性状的改良提供依据。

In this study, a Real-time PCR system was developed for detection of Keratin 26 （KRT26） gene expression difference of skin tissues in Superfine and fine Merino sheep using 18S rRNA, β-actin and GAPDH genes as internal control genes based on SYBR Green Ⅰ dying technique. The results showed that threshold cycle（Ct） of the standard curve displayed good linear relationship with the concentrations of standard eDNA in a certain range. Significant difference of KRT26 expression were observed in the skin tissues of different fineness of wool sheep, and the expression ratio between super-fine and fine wool was 1.5 when the 18S rRNA,β-actin and GAPDH were reference genes respectively. It might be sure the relationship with KRT26 and the wool fineness, and this might be provide a foundation for molecular breeding and improvement of the wool traits of sheep.