荷斯坦奶牛乳腺β-防御素的差异表达

Differential Expression of β-defensins from Mammary Gland in Holstein Cow

本研究为证明荷斯坦奶牛乳腺β-防御素的表达,扩增出6种β-防御素序列进行氨基酸分析,结果发现,扩增的6个保守的半胱氨酸,其核酸序列与NCBI数据库序列进行BLAST比对,结果LAP、BNBD4同源性100%,BNBD5为99.6%,EBD为99.5%,BNBD7、TAP为98.5%;实时荧光定量PCR检测6种防御素的mRNA水平的表达,它们之间的表达量都有显著性差异(BNBD4与BNBD5除外),其中LAP表达量最多,BNBD7,EBD,BNBD5,BNBD4次之,TAP的表达量最低。

The aim of the present study was to demonstrate the expression of β-defensins in the mammary glands of Hol- stein cows. Six kinds of β-defensins were amplified and compared the amino acid sequence. The results showed that all of them had six conserved cysteine residues. The nucleic acid sequence was compared with the NCBI database sequence, the homology result of LAP and BNBD4 were 100%, BNBD5 was 99.6%, EBD was 99.5%, BNBD7 and TAP were 98.5%. The mRNA expression of six kinds of defensins was detected by using Real-time quantitative PCR. The results showed that the relative ex- pression of them were significant diflerence each other, but only the expression of BNBD4 and BNBD5 was no significant differ- ence. The relative expression quantity of LAP was the highest, BNBDT, EBD, BNBDS, BNBD4 were middle, TAP was the lowest.