抗猪传染性胃肠炎病毒与猪流行性腹泻病毒卵黄抗体制备及其临床应用研究

Study and Application the Hyperimmunized Yolk Antibodies of TGEV and PEDV in Piglets

本研究旨在制备猪传染性胃肠炎病毒和猪流行性腹泻病毒高免卵黄抗体,研究其治疗效果。以猪传染性胃肠炎和猪流行性腹泻二联灭活苗免疫产蛋鸡,琼脂扩散方法检测抗体效价达到1∶64时,收集卵黄,采用氯仿抽提和硫酸铵盐析法纯化卵黄抗体,进行微生物学检测、安全性试验,通过人工感染治疗试验和临床应用,观察其治疗效果。结果人工感染治愈率为100%,临床应用治愈率为88.0%,表明制备的卵黄抗体对猪传染性胃肠炎和流行性腹泻具有显著的治疗效果。

The hyperimmunized yolk antibodies against porcine transmissible gastroenteritis virus （TGEV） and poricine ep- idemic diarrhoea virus （PEDV） was obtained, and its cure effect was tested. The hens were immunized with the two combined inactivated vaccine of TGEV and PEDV. After the antibody titer reached to 1 ： 64 tested by double diffusion and eggs of hyper- immunity were collected. The hyperimmunized yolk antibodies were purified by chloroform extraction and salt precipitation with ammonium sulfate. Microorganism detection, safe experiment, artificially infected piglets with the antigen and clinical treatment test were conducted to evaluate the application effect. The cure rate of artificial infection was 100 % and the cure rate of clinic treatment test was 88.0 %. The antibody had obvious curative effect against TGEV and PEDV.