种植体周龈沟液中白细胞介素-17和23质量浓度的测定

The determination on the concentration of interleukin-17 and 23 in the peri-implant sulcular fluid of patients

目的探讨白细胞介素(IL)-17和23在正常种植体和种植体周围炎中表达的异同,两者在种植体周围炎发生发展过程中的作用和相互关系。方法根据种植体周情况将其分为健康种植体组和炎症种植体组,以对侧同名健康天然牙为对照组。采集龈沟液并测量其体积,运用夹心酶联免疫吸附试验测定IL-17和23的质量浓度。结果在种植体周围炎患者龈沟液中,IL-17和23的质量浓度明显高于健康种植体组(P<0.01),健康种植体组略高于对照组(P<0.05)。种植体周和天然牙周龈沟液的体积以及IL-17和23与牙周状况呈正相关关系,种植体周围龈沟液中IL-17与23呈线性正相关。结论IL-17和23的质量浓度与种植体周围炎的发生相关,种植体周IL-17与23间的质量浓度呈正相关,即IL-17和23可能在种植体周围炎症反应过程中形成了IL-23—IL-17免疫应答途径。

Objective To explore the similarities and differences of the expression of interleukin （IL）-17 and 23 between the group of peri-implantitis and peri-implantar healthy patients. To determine the effeet and mutual relations of IL-17 and 23 in the development and progression of peri-implantitis. Methods Implant cases were divided into health-implant group and peri-implantitis group, the health of natural teeth of the opposite side with the same name as the control group. The peri-implant sulcular fluid were collected to determine the concen- tration of IL-17 and IL-23 by using enzyme-linked immunosorbent assay method. Results In the peri-implant sulcular fluid of the peri-implantitis, the concentration of IL-17 and 23 was significantly higher than that in the implant group（P〈0.01）, health-implant group was slightly higher than that in the control group （.P〈0.05）. The vo- lume of implant and natural periodontal gingival crevicular fluid, and IL-17 and 23 had a positive correlation with periodontal status. There was a significant positive correlation between the concentration of IL-17 and IL-23 in the peri-implantitis group. Conclusion The concentration of IL-17 and IL-23 were positively correlated with the incidence of peri-implantitis. IL-17 and IL-23 can form IL-23-IL-17 immune response pathway in the in- cidence of peri-implantitis.