长春花吲哚萜类生物碱代谢途径研究进展

Research Progress of Terpenoid Indole Alkaloids Metabolism Pathway in Catharanthus Roseus

长春花(Catharanthus roseus(L.)G.Don.)含有130余种生物碱,既是抗癌药物长春碱和长春新碱的唯一植物来源,也是研究吲哚萜类生物碱代谢途径的模式植物。生成长春花生物碱的前体环烯醚萜主要来源于2-C-甲基-D-赤藓醇4-磷酸途径(MEP),甲羟戊酸途径(MVA)可能在此过程中起辅助作用。吲哚生物碱代谢途径受茉莉酸甲酯正调控。文多灵途径过去被认为在长春花悬浮细胞中没有文多灵产生,而目前的研究发现文多灵在长春花C20hi细胞中可以微量合成。文章对近年来长春花吲哚生物碱代谢途径及部分关键酶研究进展进行了综述。

Catharanthus roseus （L.） can produce more than 130 alkaloids. It is the only natural resource of vinblastine and vincris- tine and the model plant to investigete the biosynthesis of terpenoid indole alkaloids （TIAs）. The MEP pathway is the major way to produce iridoid precursors and the MVA pathway maybe plays a minor role in the alkaloids metabolism. The indole pathway could be up-regulated with methyl jasmonate. It was believed that vindoline could not be yielded in cultured cells. But the recent research revealed that C. roseus C20hi cells could produce vindoline at a minor level. This paper reviews the research progress of TIAs metabo- lism and some important enzymes in the pathway in C. roseus.