RP-HPLC测定桂郁金中吉玛酮的含量

Determination of Content of Germacrone in Curcuma kwangsiensis by RP-HPLC

目的:建立RP-HPLC测定桂郁金中吉玛酮的含量。方法:采用正交试验优化样品处理的提取条件,采用RP-HPLC检测吉玛酮的含量,Phenomenex C18色谱柱(4.6 mm×250 mm,5μm),流动相76%乙腈,流速1.0 mL.min-1,检测波长为217 nm。结果:吉玛酮在0.056 7～2.324 7μg呈良好的线性关系(r=0.999 99),平均回收率98.05%,RSD 0.69%;容县产桂郁金吉玛酮含量最高,而平南产含量最低;桂郁金块根炮制品及桂郁金茎叶中吉玛酮的含量明显低于自然阴干的桂郁金块根。结论:吉玛酮含量测定方法简单、准确、快速;不同产地、不同部位以及不同炮制品的桂郁金吉玛酮含量相差很大。

Objective： To establish a HPLC method for the determination of germacrone in Curcuma kwangsiensis. Method： After using the orthogonal test to optimize extracting conditions, germacrone was separated on Phenomenex C18 （4.6 mm × 250 mm, 5 μm） column and detected at 217 nm, with 76% acetonitrile as mobile phase, and the flow rate was 1.0 mL· min^-1 Result： Germacrone was linear in the range of 0. 056 7- 2. 324 7μg （r =0. 999 99） and the average recovery of germacrone was 98.05% with RSD 0.69%. The most and the least content of germacrone in C. kwangsiensis was from Rong county and Pingnan county respectively. The germacrone in processed root tubers and stems and leaves of C. kwangsiensis were much less than that in the root tubers dried in the shade. Conclusion： The method is simple, C. kwangsiensis from different producing areas, medicinal parts accurate and rapid. The conternt of germacrone in and processed products make a great difference.