复方清开灵注射液的效应物质探索

Study on Effector Substance of Qingkailing Injection Directed by Meridian Distribution

目的:以黄芩归经为指导,探索清开灵注射液(QKL)的效应物质。方法:取大鼠,给药后于不同时间点处死,取脑、肺、肝。另取大鼠,分为正常组和缺血再灌注组,各组大鼠按采样点随机分为5组,每组5只。正常组大鼠经尾静脉给予800 mg.kg-1QKL。脑缺血再灌注组采用改良的腔内线拴法通过阻闭大脑中动脉120 min后再灌注120 min复制局灶性脑缺血-再灌注病理模型,并于恢复再灌注120 min时给予800 mg.kg-1QKL。给药后于不同时间点处死,取脑、肺、肝。HPLC-MS法测定黄芩苷浓度。结果:正常大鼠注射清开灵后,大量黄芩苷很快分布至肺、肝中,脑中分布较少。脑缺血-再灌注大鼠脑中黄芩苷量较正常组明显升高。结论:黄芩苷为清开灵治疗肝、肺、脑缺血疾病的效应物质之一;以中药归经为指导,进行复方效应物质探索具有可行性。

Objective： Directed by meridian distribution of Radix Scutellariae, studying effector substance of Qingkailing injection. Method： Qingkailing injection was given to experimental rats, the brain, liver and lung were collected in various time. Then Qingkailing was injected to both normal and cerebral isehemia- reperfusion rats, the brain, liver and lung were collected at various time. The concentration of baicalin from samples was determined by HPLC-MS. Result： After iv Qingkailing injection, considerable baicalin was distributed quickly to target tissue of normal rats, ie liver and lung; the content of baicalin in the brain was minimum. In cerebral ischemia-reperfusion rats, the concentration of baicalin in the brain was higher than that of normal rats. Conclusion： Baicalin is one of effector substance of Qingkailing injection curing lung, liver and cerebral ischemia disease. It is feasibility to search for effector substance of complex prescription, directed by meridian distribution of traditional Chinese medicine.