钩藤散对AD模型大鼠海马CA1区相关指标的影响

The Effect of Gouteng San on the Related Indicators on the Hippocampal CA1 Area in Alzheimer's Disease Model Rat

目的:观察钩藤散对阿尔茨海默病(AD)模型大鼠海马CA1区相关指标的影响。方法:用β-淀粉样蛋白(Aβ1-40)海马注射建立AD大鼠模型,然后分成钩藤散高、中、低剂量、模型、正常对照5个组,钩藤散分别用5,2.5,1.25 g.kg-1ig AD大鼠,每天1次,连续给药15 d,14 d时迷宫法测定其行为学指标,未次给药后1 h将动物麻醉、心脏灌注、取脑、制作海马CA1区病理切片,用免疫组化法测定神经细胞数,Aβ,突触后密度蛋白-95(PSD-95)阳性细胞数和平均吸光度(A)。结果:行为学测试成绩:对照组、各给药组均好于模型组(P<0.01),说明模型制作成功并提示钩藤散有益智作用;CA1区的神经细胞数、PSD-95阳性细胞数和平均A:各给药组均高于模型组(P<0.001);Aβ阳性细胞数和平均A:各给药组均低于模型组(P<0.01),说明钩藤散能有效降低脑组织Aβ含量、提高PSD-95水平,保护神经细胞。结论:钩藤散对AD模型大鼠海马组织有一定保护作用。

Objective： To observe the regulatory effect of Gouteng San on the related indicators on the hippocampal CA1 area in Alzheimer＇s disease （AD） model rat. Method： AD rat model was established by hippocampal injection of β-amyloid protein （Aβ1.40） , and then divided into high, meddlu and low-dosage, the model and normal control group. Oral administration of Gouteng San （5, 2.5, 1.25 g·kg^-1） was started for 15 constitutive days. The maze behavioral indicators were measured at day 14. After administration of Gouteng San for 1 h, the rats were given anesthesia and cardiac perfusion to obtain the brain, make hippocampal CA1 district biopsy, and immunohistochemical determination was used to assay nerve cells, Aft, postsynoaptic density-95 （PSD-95） positive cells and mean optical density value. Result： After the treatment of Gouteng San, behavioral test results showed that that Gouteng San could improve the learning and memory ability in the rats. The nerve ceils in CA1 region, PSD-95 positive cells and average optical density values were higher than model group （P 〈 0. 001 ）, Aft positive cells and the mean optical density values were lower than the model group （ P 〈 0.01 ）, indicating that Gouteng San could effectively reduce Aft levels in the brain tissue while increasing PSD-95 positive cells and protecting nerve cells. Conclusion： Gouteng San has a protective effect on the hippocampus area in AD rats.