清肝调脂饮抗非酒精性脂肪性肝炎大鼠脂质过氧化反应的研究

Effect of Qinggan Tiaozhi Decoction on Lipid Peroxidatic in Rat Nonalcoholic Steatohepatitis

目的:探讨清肝调脂饮对非酒精性脂肪性肝炎(NASH)大鼠脂质过氧化反应的干预机制。方法:Wistar大鼠70只,除10只作为正常对照组外,余60只建立以高脂饲料(基础饲料88%,猪油10%,胆固醇1.5%,胆盐0.5%)诱导的非酒精性脂肪性肝炎大鼠模型并随机分为5组,除模型组外,造模同时ig清肝调脂饮低、中、高剂量组(4.59,9.18,18.39 g.kg-1)和西利宾胺组(27 mg.kg-1,ig)分别按临床等效剂量的0.5,1,2和1倍剂量药物治疗。实验12周,大鼠全部处死。检测各组大鼠血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),甘油三酯(TG)含量,肝组织匀浆肿瘤坏死因子(TNF-α),丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性;肝组织行CD14,细胞色素P450 2E1(CYP2E1)免疫组化染色,阳性结果作定量分析。结果:与空白对照组比较,模型组ALT(62.57±17.08)U.L-1,TNF-α(902.00±22.66)pg.mg-1,CYP2E1阳性率(0.88±0.07),SOD(81.78±2.24)U.mg-1各指标均显示异常(P<0.05,P<0.01);与模型组比较,各用药组均能不同程度的改善以上各指标(P<0.05,P<0.01)。在降低TG,TNF-α方面,清肝调脂饮高剂量组明显优于西药对照组(P<0.01);而对其他各指标的改善,清肝调脂饮各剂量组与西药对照组疗效无显著差异。结论:清肝调脂饮具有降低脂质过氧化反应、提高抗氧化能力的作用,从而减轻NASH肝细胞变性坏死程度。

Objective： To observe the effect of Qinggan Tiaozhi decoction on lipid peroxidatic reaction in rat nonalcoholic steatohepatitis （NASH）. Method： The NASH model was induced by high fat diet, and the modeling rats were randomly divided into five groups： the model group, the low dose, middle dose and high dose group of Qinggan Tiaozhi decoction and the group of silybin, each group were treated with corresponding 4.59, 9.18, 18.39 g·kg^-1 and 27 mg·kg^-1, ig. The indexes of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and triglyceride （TG） in serum were detected, the levels of tumor necrosis factor alpha （TNF-α） , malondialdehyde （MDA） and superoxide dismutase （SOD） in liver tissue homogenate were measured, CD14 and cytochromes P450 2El （CYP2E1） in liver tissue for quantitative analysis were assayed by immunohistochemical stain. Result： The indexes of ALT, TNF-α, CYP2E1 and SOD in model group were changed compared with those in normal group （ P 〈 0.05, P 〈 0.01 ） , otherwise they were all improved in treated groups than those in model group （P 〈0.05, P 〈0.01）. The indexes of TG and TNF-α were improved in high dose group of Qinggan Tiaozhi decoction than that in silybin group （P 〈 0.01 ） , and for other indexes, there were similar effects between Qinggan Tiaozhi decoction and silybin group. Conclusion： Compared with silybin, Qinggan Tiaozhi decoction has better effects on NASH.