中药止痛巴布贴对骨癌痛大鼠脊髓背角p-ERK、p-CREB的影响

Influence of Pain-Relieving Plaster on Expressions of pERK and pCREB in Spinal Dorsal Horn of Rats with Bone Cancer Pain

目的:观察中药止痛巴布贴对骨癌痛大鼠痛行为及骨髓背角神经节pERK、pCREB表达的影响。方法:108只180～220g雌性Wistar大鼠随机分为3组,分别为空白对照组(Con组,n=24)、假手术组(Sham组,n=24)、骨癌痛模型组(Ca组,n=60),3组分别于手术前2天、术后每隔4日测定机械痛阈(MWT)和热痛阈(TWL)。于术后7天确认造模成功后,将成功的模型鼠随机分为两组,即模型组(Ca组,n=28)和中药止痛巴布贴组(CM组,n=25)。术后7天、14天和21天各组处死大鼠(n≥8),取大鼠脊髓腰椎L4-6膨大处,用免疫组化方法测定脊髓背角pERK、pCREB的变化。结果:脊髓背角神经pERK、pCREB表达:Ca组阳性神经元数目增加,术后7天、术后14、21天与Con组和Sham组比较有统计学差异(P<0.05);CM组于术后14天、术后21天与Con组和Sham组统计学差异逐渐缩小(P>0.05)。结论:中药止痛巴布贴对骨癌痛有比较明显的镇痛作用,其对脊髓背角c-fos的影响,可能是通过降低脊髓背角神经节pERK、pCREB的表达而产生的,即通过ERK-CREB信号转导通路完成的。

Objective ： The influence of Pain - Relieving Plaster on expressions of pERK and pCREB in the spinal dor- sal horn of rats with bone cancer pain. Method： 108 female Wistar rats （180- 220g）were randomly divided into 3 groups, respectively ：blank control group （group Con, n = 24）, sham operation group （group Sham, n = 24）, bone cancer pain model group （ group Ca, n = 60）. The 3 groups were determined mechanical pain threshold （MWT） and thermal pain threshold（TWL）2 days before operation and every 4 days after operation. On the 7th day after operation, the successful model rats were randomly divided into two groups：the model group （group Ca, n = 25）and Pain - Relieving Plaster group （group CM, n = 18 ）. After 7 days, 14 days and 21 days rats in each group were sacrificed （ n ≥ 8）, the determination of pERK and pCREB in spinal dorsal horn of L4 - 6 was modified by immunohistochemical method. Result ： Spinal dorsal horn pERK and pCREB expression：number of positive neurons in group Ca increased, which had stastistieal difference compared with group Sham and group Con 7,14,21 days after operation（ P 〈 0.05 ） ; 14,21 days after operation, compari- son difference between group CM and group Con or group Sham were narrowed gradually（ P 〉 0.05 ）. Conclusion： Pain - Relieving Plaster has obvious analgesic effect on bone cancer pain, possibly by reducing pERK and pCREB expressionsof spinal dorsal horn which is completed by ERK - CREB signal transduction pathway.